产品货号：
Z21702
中文名称：
NOTCH1抗体
英文名称：
Anti-NOTCH1 Antibody
产品规格：
200μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NOTCH1 Antibody |
| 指标别名 | hN1;Notch 1;NOTCH1;TAN1 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse |
| 应用范围 | Dot blot,ELISA,IHC,WB |
| 基因名称 | NOTCH1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). |
| 计算分子量 | 273kDa |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.1% (w/v) Sodium Azide |
| 纯化方式 | This antiserum is directed against human NOTCH 1.Based on the immunogen sequence,we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology).This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells.Only the cleaved intracellular (activated) form (NICD) is detected.No reactivity is detected against mouse N2,N3 or N4.The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. |
| 浓度 | 90mg/mL by Refractometry |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Anti-Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active,ligand-accessible form.Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC).Following ligand binding,it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. |
| Uniprot ID | P46531 |
| 基因名全称 | Neurogenic locus notch homolog protein 1 |
| 推荐稀释比 | ELISA:1:20,000 - 1:60,000 IHC:1:200 IF Microscopy:User Optimized IP:User Optimized WB:1:500- 1:2,000 |
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